Effect of L.plantarum on Improvement Process of Gastric Inflammation of H.pylori infection in Animal Model C57BL/6
Authors
-
Behnoush Asgari 1
1- Dept. of Microbiology, Student Research Committee, Alborz University of Medical Sciences, Karaj, Iran.
http://orcid.org/0000-0001-8423-0652
- Fatemeh Kermanian 2 2- Dept. of Anatomy, Alborz University of Medical Sciences, Karaj, Iran.
- Farzad Khalili 3 3- Dept. of Gastroenterology, Alborz University of Medical Sciences, Karaj, Iran.
- Zahra Rohani Nojede Sadat 4 4- Student in Medical School, Alborz University of Medical Sciences, Karaj, Iran.
- Somaye Yaslianifard 5 5- Dept. of Microbiology, Alborz University of Medical Sciences, Karaj, Iran.
DOI:
https://doi.org/10.22100/jkh.v13i2.1879Keywords:
Helicobacter pylori, Lactobacillus plantarum, Gastric ulcer, Histopathology, C57BL/6.Abstract
Introduction: Helicobacter pylori has become accepted as an important human pathogen for the development of gastritis since it was first reported by marshal and warren in 1983. About half of the world human population is infected by H. pylori. Current standard therapies against H. pylori are based on the use of one proton pump inhibitor plus two or more antibiotics. The efficacy of this treatments has been decreased, mainly due to the increase of antibiotic resistance but also to side effects. So we tried to follow other therapies. The role of probiotics in the treatment of gastrointestinal infections has increased during recent decades. One of the most important groups of probiotics is lactic acid bacteria. The aim of this study was to analyse the efficacy of Lactobacillus plantarum on gastric ulcer induced by helicobacter pylori (ATCC43504).
Methods: In this experiment, the mice were randomly divided into three experimental groups (ten mice in each group). Mice were inoculated with h.pylori suspension (5*1010cfus/ml) in PBS by gavage twice daily for three consecutive days. Four weeks after the last inoculation we confirmed the infection by determination of h.pylori stool Ag (ELISA) and histopathological examination. One mouse from each group sacrificed and the tissues removed. The groups were subjected to different therapies as stated, 1: without Bismuth (Bi) and Omeprazole (Om) prescription, 2: Bi, Om and Clarithromycin (Cl) and 3: Bi, Om plus 1cc of suspension of 109CFU/ml of L. plantarum. After 2 weeks, the stool was analyzed for Ag and the mice were sacrificed for evaluation of histopathologic changes.
Results: Treatment with L. planratum group provided Zero titer of stool Ag and was associated with improved gastric inflammation in all subjects, similar to the clarithromycin group
Conclusion: L.plantarum probiotics provides similar results as clarithromycin in terms of improvement of H. pylori infection and gastritis in C57BL/6 Mice model, without its cons of antibiotic resistance.References
Hunt R, et al. Helicobacter pylori in developing countries. J Gastrointestin Liver Dis 2011;20:299-304.
Owens SR, Smith LB. Molecular aspects of H. pylori-related MALT lymphoma. Pathology research international 2011;2011.
Javani S, Mehryari A. The Role of probiotics in the treatment and prevention of H. pylori Infection. EBNESINA 2007;9:24-8.
Santos AM, Lopes T, Oleastro M, et al. Curcumin inhibits gastric inflammation induced by Helicobacter pylori infection in a mouse model. Nutrients 2015;7:306-20.
Gene E, et al. Triple vs. quadruple therapy for treating Helicobacter pylori infection: a meta‐analysis. Alimentary pharmacology & therapeutics 2003;17:1137-43.
Wang Y-h, Huang Y. Effect of Lactobacillus acidophilus and Bifidobacterium bifidum supplementation to standard triple therapy on Helicobacter pylori eradication and dynamic changes in intestinal flora. World Journal of Microbiology and Biotechnology. 2014;30:847-53.
Patel A, Shah N, Prajapati J. Clinical application of probiotics in the treatment of Helicobacter pylori infection-a brief review. Journal of Microbiology, Immunology and Infection 2014;47:429-37.
Weizman Z. Effect of probiotic infant formulas on protection from infection in child care centers. BioGaia 2008;42:1-12.
Demirer S, et al. Effects of probiotics on radiation-induced intestinal injury in rats. Nutrition 2006;22:179-86.
Guslandi M, et al. Saccharomyces boulardii in maintenance treatment of Crohn’s disease. Digestive diseases and sciences 2000; 45:1462-4.
Chen X, et al. Antagonistic activities of lactobacilli against Helicobacter pylori growth and infection in human gastric epithelial cells. Journal of food science 2012;77.
Hsieh PS, et al. Eradication of Helicobacter pylori Infection by the Probiotic Strains Lactobacillus johnsonii MH‐68 and L. salivarius ssp. salicinius AP‐32. Helicobacter 2012;17:466-77.
Kabak B, et al. Effects of probiotic bacteria on the bioaccessibility of aflatoxin B1 and ochratoxin A using an in vitro digestion model under fed conditions. Journal of Environmental Science and Health, Part B 2009;44:472-80.
Zambon C-F, et al. Non-invasive diagnosis of Helicobacter pylori infection: simplified 13C-urea breath test, stool antigen testing, or DNA PCR in human feces in a clinical laboratory setting? Clinical biochemistry 2004;37:261-7.
Hamilton-Miller J. The role of probiotics in the treatment and prevention of Helicobacter pylori infection. International journal of antimicrobial agents 2003;22:360-6.
Nakajima H, Suzuki Y, Hirota T. Cholesterol lowering activity of ropy fermented milk. Journal of food science, 1992;57:1327-9.
Friedman Lawrences, P.W. and L.H. S, Principles of internal medicin. New york Mc GRAW Hill, 1998. 14 th(10): p. 1602- 1604.
Schiraldi C, et al. Exopolysaccharides production in Lactobacillus bulgaricus and Lactobacillus casei exploiting microfiltration. Journal of Industrial Microbiology and Biotechnology, 2006;33:384-90.
Kaur I, et al. Entrapment of probiotic bacteria within alginate-HPMC floating beads, their survival and effectiveness against ethanol induced ulcers in rats. International Journal of Probiotics And Prebiotics 2007;2:41.
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