Effect of Chronic Administration of Melatonin on Testicular Damage in Streptozotocin- Induced Diabetic Male Rat
DOI:
https://doi.org/10.22100/jkh.v7i3.149Keywords:
, Diabetes, Melatonin, Spermatogenesis, Sertoli cell, Testes injuryAbstract
Introduction: Diabetes malady because of making oxidative stress has an important role in causing the metabolism damages. The aim of this study was to assess the effect of melatonin with regard to its anti oxidant attribute for treatment of testicular damage of diabetes.
Methods: In this research, 24 male vistar rats with average weight of 200-220g were choosen and divided into 3 groups of control, diabetic and treatment. Diabetes group was induced by Sterptozotosin (STZ) injection(55mg/kg), and treatment group, (diabetic + melatonin), after two months of becoming diabetic received melatonin pill with 10mg/kg dose of it in gavage way during 5 weeks.
At the start of the fifth week, the blood samples were taken from the rats and biochemical and blood hormones invoices and also testicle sections were histologically evaluated.
Results: Diabetes through producing oxidative stresses and producing of free radicals decreases spermatogoni and sertoly cell and also decreases spermatogenesis and sperm functions. Diabetic also decreases quantity of insulin and testosterone hormones. Melatonin administration increases amount of plasma insulin and testosterone hormone that significantly decreases the produced morphologic changes.
Conclusion: Melatonin can prevent and cure oxidative stresses and also it can be a cure for testicular damages resulting from diabetes.
References
Sudnikovich E, Maksimchik Y, Zabrodskaya S, Kubyshin V. Melatonin attenuates metabolic disorders due to streptozotocin induced diabetes in rat. European Journal of pharmacology 2007;180-87.
Guneli E, Tugyan K, Ozturk H, Gumustekin M, Cilaker S, Uysal N. Effect of melatonin on testicular damage in streptozotocin induced diabetes rats: Eur surg res 2008;40:354-360.
Scarano WR, Messias AG, Oliva SU, Klinefelter GR, Kempinas WG. Sexual behavior, sperm quantity and quality after short –term sterptozotocin induced hyperglycaemia in rats. Int J Androl 2006;29:482-488.
Seithikurippu R, Trakht I, Srinivasan V, Warren Spence D, et al. Physiological effect of melatonin: role of melatonin receptors and signal transduction pathways. Progrees in Neurobiology 2008;85:335-53.
Abdolah Nezhad A, Gol A, Dabiri SH, Javadi AD. Effect of allium sativum on testicular damage in streptozotocin induced diabetes rats. Iranian Journal of Endocirinology and Metabolism Services 2009;11(4):443-53.[Persian].
Becker A, Wiesenberg I, Schaeren W, Missbach M, Saurat JH, Carlberg C. Pineal gland hormone melatonin binds and activates an orphan of nucleur receptor super family. Journal of Biology 1994; 269:28531-34.
Reiter RJ. Melatonin: lowering the high price of free radicals. News Physiol Sci 2000;15:246-250.
Cameron DF, Murray FT, Drylie DD. Interstitial compartment pathology and spermatogenic disruption in testes form impotent diabetic men. Anat Rec 1985;213:53-62.
Babaei Balderlou F, Ilkhanipour M, Heidari R, Zare S, Bernousi I. Effect of melatonin on peripheral Neuropathic pain in diabetic rat. Iranian Journal of Endocirinology and metabolism Services 2009; 11(1):79-87.[Persian].
Paskologlu k, Sener G, Ayangolu Dulger G. Melatonin treatment protects against diabetes induced functional and biochemical changes in the rat aorta corpus cavermosum. European Journal of Pharmacology 2004;499:345-54.
Martinez Cruz F ,Guerrero JM, Osuna C. Melatonin prevents the formation of pyrrolized proteuns in human plasma induced by hydrogen peroxide. Neurosci Lett 2002;326:147-50.
Nishidia S. Metabolic effects of melatonin on oxidative stress and diabetes mellitus. Endocrine 2005;27:131-36.
Cai L, Hales BF, Robaire B. Induction of apoptosis in the germ celld of adult male rats after exposure to cyclophosphamide. Boil Reprod 1997;56(6):1490-7.
Hussein MR, Abu Dief EE, Abd EI-Reheem MH, Abd Elahman A. Ultrastructural evaluation of the radioprotective effects of melauation against X-ray –induced skin damage in Albino rats. Int Exp Pathol 2005;86:45-55.
Cai L, Chen S, Evans T, Deng DX, Mukherjee K, Chakrabarti S. Apoptptic germ –cell death and testicular damage in experimental diabetes: prevention by endothelin antagonism. Urol Res 2000; 28:342-47.
Balasinorn NH, Dsouzar U. Effect of high intratesticular estrogen on spermatogenesis. International Journal of Fertility and Sterility 2010;
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