The Effect of Eight Weeks of Endurance Training and Injection of Growth Hormone Lipolytic Fragment (AOD9604) on CK18 and Liver Enzymes of NAFLD-Induced Mice Induced by High-Fat Diet

Abstract

Introduction: Fatty liver is on the rise in the world, so the aim of this study was to investigate The effect of eight weeks of endurance training, injection of growth hormone lipolytic fragment (AOD9604) on CK18 and liver enzymes of NAFLD on mice induced mice induced by high-fat diet.

Methods: In This experimental study, 28 male mice were, divided into four groups(n=7): control (C), Exercise (E), Exercise + Fragment (EA), Fragment without training (A) group. The Medium intensity endurance training program was performed for eight weeks and 5 sessions per week. The Fragment injection protocol was considered to be 250 picograms per kilogram of body weight per day. Samples were evaluated 48 hours after the last training  session . Statistical analysis of the data using the software SPSS version 26, using OneWay ANOVA and post hoc Tukey tests at the significant level (P<0/05) was carried out.

Results: CK18 showed a significant decrease compared to the control group only in group E(P=0.00). HOMA-IR index had a significant decrease in E (P=0.00) and EA (P=0.03) groups, but no significant changes in A group. Changes in AST were not significant in any of the groups. ALT levels in all three groups of E (P=0.00), A(P=0.00) and EA(P=0.00) were significantly lower than the control group.

Conclusion: Despite not having a negative effect on AOD9604, endurance training alone showed a better response to this peptide. It seems that fragmentation along with exercise can be effective in improving fatty liver syndrome.