Protective Effects of Pentoxifylline on Methamphetamine Induced Cell Death in PC12 Cells

Authors

DOI:

https://doi.org/10.22100/jkh.v18i1.3076

Keywords:

Methamphetamine, Pentoxyphylline, Apoptosis, Cell death

Abstract

Introduction: Methamphetamine abuse has been a global concern in the last few decades. An estimated 3.5 million people have been affected by methamphetamine abuse. Methamphetamine induces apoptosis in most cell lines. Pentoxyphilline, as a phosphodiesterase inhibitor, can reduce methamphetamine-induced cell death by inflammation reduction.

Methods: PC12 cells were grown in a DMEM culture medium. Assays used in this study are listed below: MTT test for cell viability detection, LDH test for cytotoxicity measurement, caspase activity colorimetric assay kit (Bio-techne) for caspase-3 activity diagnosis, Rhodamine 123 for detection of mitochondrial membrane potential, fluorescence microscope for measurement of antioxidant enzyme activities.

Results: Pentoxyphilline increased cell viability and the Rhodamine-123 absorbance. Besides, it reduced cell cytotoxicity, caspase-3 activity, and (OH) generation in all concentrations of 1 nM to 100 µM (P<0.05) by an optimal concentration of 100 µM.

Conclusion: In conclusion, Pentoxyphilline, as a phosphodiesterase inhibitor, can significantly reduce methamphetamine-induced cell death through its anti-inflammatory effects.

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2023-11-27

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Protective Effects of Pentoxifylline on Methamphetamine Induced Cell Death in PC12 Cells. (2023). Knowledge and Health in Basic Medical Sciences, 18(1), Page:60-67. https://doi.org/10.22100/jkh.v18i1.3076

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