بررسی سطح بیان BDNF در موش‌های نر ویستار پس از شش هفته شنا

نویسندگان

DOI::

https://doi.org/10.22100/jkh.v19i3.3259

کلمات کلیدی:

فاکتور نوروتروفیک مغزی, هیپوکامپ و دمای آب.

چکیده

مقدمه: تمرینات هوازی موجب بهبود در عملکرد شناختی میشوند. با توجه به نقش کلیدی BDNF در بهبود عملکرد شناختی، پژوهش حاضر، اثر شش هفته شنا در آب سرد و دمای معمول را بر عمکرد شناختی و بیان BDNF بافت مغز مورد بررسی قرار داده است.

مواد و روش‌‌‌ها: 25 سرموش نر ویستار (8 هفتهایی، وزن 30±250) از مؤسسه پاستور خریداری و پس از دو هفته آشناسازی با محیط استاندارد آزمایشگاه، در آزمون اولیه عملکرد شناختی شرکت کردند و سپس بهطور تصادفی به سه گروه شنا در آب سرد و دمای معمول و کنترل تقسیم شدند. هر سه گروه آزمون اولیه عملکرد شناختی را اجرا کردند. سپس دو گروه تمرین، شش هفته پروتکل تمرین را اجرا کردند. 48 ساعت پس از آخرین جلسهی تمرین، موشهای صحرایی قربانی شدند و بافت مغز آنها برداشت شد. سپس برای سنجش بیان ژن BDNF از روش ریل تایم PCR استفاده شد. برای تحلیل یافتهها نیز از آزمون آنووای یکطرفه استفاده شد.

نتايج: نتایج نشان داد، عملکرد شناختی در پاسخ به شش هفته شنا کردن نسبتبه گروه کنترل، بهبودیافته است (001>P). همچنین سطوح بیان BDNF بافت مغزی در دو گروه تمرین افزایش معنادار نسبتبه گروه کنترل داشت (002>P). علاوه براین تغییرات بیان BDNF گروه شنا در آب سرد به لحاظ آماری بیشتر از گروه شنا در آب با دمای معمولی بود (04/0>P).

نتیجه‌گیری: بهنظر میرسد تغییرات بیان BDNF مسئول بخشی از بهبود عملکرد شناختی در پاسخ به تمرینات ورزشی است و شنا در آب سرد میتواند محرک مناسبی برای اثربخشی بیشتر تمرین بر BDNF داشته باشد.

مراجع

Wrann CD WJ, Salogiannnis J, Laznik-Bogoslavski D, Wu J, Ma D, Lin JD, Greenberg ME, Spiegelman BM. . Exercise induces hippocampal BDNF through a PGC-1alpha/ FNDC5 pathway. Cell Metab. 2013;18(5):649–59.

Sun G QS, Jiang Q, Liu K, Li B, Li M, Zhao L, Zhou Z, von Deneen KM, Liu Y. Hyperthermia-induced disruption of functional connectivity in the human brain network. PLoS One. 2013;8(4):e61157.

Smiley-Oyen AL LK, Francois SJ, Kohut ML, Ekkekakis P. Exercise, fitness, and neurocognitive function in older adults: The “selective improvement” and “cardiovascular fitness” hypotheses. Ann Behav Med. 2008;36(3):280–91.

Chaddock L HC, Buck SM, Cohen NJ. Aerobic fitness and executive control of relational memory in preadolescent children. Med Sci Sports Exerc. 2011;43(2):344–9.

Chaddock L EK, Prakash RS, Voss MW, Van- Patter M, Pontifex MB, Hillman CH, Kramer AF. A functional MRI investigation of the association between childhood aerobic fitness and neurocognitive control. Biol Psychol. 2012;89(1):260–8.

Raine LB L, Saliba BJ, Chaddock-Heyman L,Hillman CH, Kramer AF. The influence of childhood aerobic fitness on learning and memory. PLoS One. 2013;8(9):e72666.

Wu CT PM, Raine LB, Chaddock L, Voss MW, Kramer AF, Hillman CH. Aerobic fitness and response variability in preadolescent children performing a cognitive control task. Neuropsychology. 2011;25(3):333–41.

Hansen DM HS, Lambourne K, Lee J, Donnelly JE. Linear/nonlinear relations of activity and fitness with children’s academic achievement. Med Sci Sports Exerc. 2014;46(12):2279–85.

Voss MW HS, Prakash RS, Erickson KI, Alves H, Chaddock L, Szabo AN, Mailey EL, Wojcicki TR, White SM, et al. . The influence of aerobic fitness on cerebral white matter integrity and cognitive function in older adults: Results of a one-year exercise intervention. Hum Brain Mapp. 2013;34(11):2972–85.

Erickson KI WA, Sutton BP, Prakash RS, Voss MW, Chaddock L, Szabo AN, Mailey EL, White SM, Wojcicki TR, et al. . Beyond vascularization: aerobic fitness is associated with N-acetylaspartate and working memory. Brain Behav. 2012;2(1):32–41.

Ahmadiasl N AH, H€anninen O. Effect of exercise on learning, memory and levels of epinephrine in rats’ hippocampus. J Sports Sci Med. 2003;2(3):106–9.

Gordon BA RE, Brumback CR, Lee Y, Elavsky S, Konopack JF, McAuley E, Kramer AF, Colcombe S, Gratton G, et al. . Neuroanatomical correlates of aging, cardiopulmonary fitness level, and education. Psychophysiology. 2008;45(825–38):825–38.

Clark RE BN, Squire LR. Hippocampus and remote spatial memory in rats. Hippocampus. 2005;15(2):260–72.

Cotman C.W. CC, Berchtold N.C. Exercise: a behavioral intervention to enhance brain health and plasticity. Trends Neurosci. 2002;25(6):295–301.

Meiri AKaN. Brain-Derived Neurotrophic Factor Is Critically Involved in Thermal-Experience-Dependent Developmental Plasticity. The Journal of Neuroscience. 2006;26(15):3899-907.

Erickson KI VM, Prakash RS, Basak C, Szabo A, Chaddock L, KimJS, Heo S, Alves H,White SM, et al. Exercise training increases size of hippocampus and improves memory. Proc Natl Acad Sci USA. 2011;108(7):3017–22.

Rasmussen P BP, Adser H, Pedersen MV, Leick L, Hart E, Secher NH, Pedersen BK, Pilegaard H. Evidence for a release of brain-derived neurotrophic factor from the brain during exercise. Exp Physiol. 2006;94(10):1062–9.

Christopher W. Collins RJS, Matthew W. S. Heesch, and Dustin R. Slivka. The effect of environmental temperature on exercise-dependent release of brain-derived neurotrophic factor. TEMPERATURE. 2017;4(3):305–13.

Rasmussen P BP, Adser H, Pedersen MV, Leick L, Hart E, Secher NH, Pedersen BK, Pilegaard H. . Evidence for a release of brain-derived neurotrophic factor from the brain during exercise. . Exp Physiol. 2009;4(10):1062–9.

Klein AB WR, Santini MA, Clemmensen C, Ettrup A, Rios M, Knudsen GM, Aznar S. . Blood BDNF concentrations reflect brain-tissue BDNF levels across species. Int J Neuropsychopharmacol. 2011;14(3):347–53.

Nakagawa T O-KM, Sugaru E, Yamanaka M, Taiji M, Noguchi H Brain-derived neurotrophic factor (BDNF) regulates glucose and energy metabolism in diabetic mice. Diabetes Metab Res Rev. 2002;18(3):185–91.

AE J. Modulation of carbohydrate and fat utilization by diet, exercise and environment. Biochem Soc Trans. 2003;31(Pt 6):1270–3.

SR MR. The neuropharmacological and neurochemical basis of place learning in the Morris water maze. Brain Res Brain Res Rev. 1993;18(1):33-49.

Petzinger GMF BEA, G.; Holschneider, D.P.; Wood, R.; Walsh, J.P.; Lund, B.; Meshul, C.; Vuckovic, M.; Jakowec,M.W The role of exercise in facilitating basal ganglia function in Parkinson’s disease. Neurodegener Dis Manag. 2011;1:157-70.

Sara Shams MA SA-S, Hamid Rajabi , Katsuhiko Suzuki. . Swimming in cold water upregulates genes involved in thermogenesis and the browning of white adipose tissues. Comparative Biochemistry and Physiology. 2023;Part B 265:110834.

Kirk-Sanchez NJM, E.L. Physical exercise and cognitive performance in the elderly: Current perspectives. Clin Interv Aging. 2013;9:51–62.

Kim GHI, K.; Kwon, H.; Seo, S.W.; Ye, B.S.; Cho, H.; Noh, Y.; Lee, J.M.; Kim, S.T.; Park, S.E.; et al. . Higher Physical Activity Is Associated with Increased Attentional Network Connectivity in the Healthy Elderly. Front Aging Neurosci. 2016;8:198.

Spaniol J. Event-related fMRI studies of episodic encoding and retrieval: Meta-analyses using activation likelihood estimation. Neuropsychologia. 2009;47:1765–79.

Bherer LE, K.I.; Liu-Ambrose, T. A Review of the Effects of Physical Activity and Exercise on Cognitive and Brain Functions in Older Adults. J Aging Res. 2013;2013:1-8.

Cotman CWB, N.C.; Christie, L.A. . Exercise builds brain health: Key roles of growth factor cascades and inflammation Trends Neurosci. 2007;30:464–72.

Park HP, M.M. Neurotrophin regulation of neural circuit development and function. Nature reviews Neuroscience. 2013;14(7-23).

Egan MFK, M.; Callicott, J.H.; Goldberg, T.E.; Kolachana, B.S.; Bertolino, A.; Zaitsev, E.; Gold, B.; Goldman, D.; Dean, M.; et al. The BDNF val66met polymorphism affects activity-dependent secretion of BDNF and human memory and hippocampal

function. Cell 2003;112:257–69.

Kesslak JPS, Vannarith Choi, James Cotman, Carl W. Gomez-Pinilla, Fernando. Learning upregulates brain-derived neurotrophic factor messenger ribonucleic acid: A mechanism to facilitate encoding and circuit maintenance? Behavioral Neuroscience,. 1998;112(4):1012-9.

Qin WS, L.; Cao, J.; Peng, Y.; Collier, L.; Wu, Y.; Creasey, G.; Li, J.; Qin, Y.; Jarvis, J.; et al. . The central nervous system (CNS)-independent anti-bone-resorptive activity of muscle contraction and the underlying molecular and cellular signatures. J Biol Chem. 2013;288:13511–21.

Choi SHB EC, Z.K.; Lee, S.W.; Pulli, B.; Clemenson, G.D.; Kim, E.; Rompala, A.; Oram, M.K.; Asselin, C.;, al. e.. . Combined adult neurogenesis and BDNF mimic exercise effects on cognition in an Alzheimer’s mouse model. Science. 2018;36(eaan8821).

D N. Short-term memory and long-term memory are still different. Psychol Bull. 2017;143:992–1009.

Colcombe SJE KIS, P.E.; Kim, S.J.; Prakash, R.; McAuley, E. Aerobic Exercise Training Increases Brain Volume in Aging Humans. J Gerontol Ser A Biol Sci Med Sci. 2006;61:1166–70.

Quindry JCF BA. Exercise Preconditioning as a Cardioprotective Phenotype. Am J Cardiol. 2021;148:8–15.

Claudia Camerino* EC, Roberta Caloiero, Adriano Fonzino, Mariarosaria Carratù, Marcello D. Lograno and Domenico Tricarico. Evaluation of Short and Long Term Cold Stress Challenge of Nerve Grow Factor, Brain-Derived Neurotrophic Factor, Osteocalcin and Oxytocin mRNA Expression in BAT, Brain, Bone and Reproductive Tissue of Male Mice Using Real-Time PCR and Linear Correlation Analysis. frontiers in physiology. 2018;8(1101):1-8.

Mustroph ML CS, Desai SC, Cay EB, DeYoung EK, Rhodes JS. . Aerobic exercise is the critical variable in and enriched environment that increases hippocampal neurogenesis and water maze learning in male C57BL/6J mice. Neuroscience. 2012;219:62–71.

دانلود

چاپ شده

2024-10-23

شماره

نوع مقاله

مقاله پژوهشي

مقالات بیشتر خوانده شده از همین نویسنده

<< < 22 23 24 25 26 27 28 29 30 31 > >>