Comparing the Expression Level of Vegf-A Gene in Adenoma Polyps Located in Colon and Rectum

Abstract

Introduction: Colorectal cancer mostly be derived from adenomatous polyposis including tubular, villous and tubulovilluos types. Investigation of some genes that play role in angiogenesis can be used as a biomarker in molecular diagnosis for polyp progress to malignancy. The aim of this study is Comparing the expression level of VEGF-A gene in adenoma polyps located in colon and rectum and it's relation with malignancy incidence.

Methods: In this study, 50 biopsy samples of adenoma polyp and 20 normal samples were collected from Taleghani hospital. Clinical information was collected. mRNA extraction and cDNA synthesis were performed. Gene expression was investigated by Real time PCR method and fold change of gene expression was evaluated by (2-^-∆∆ct) method. The outcomes were analyzed by the ABI 7500 Sequence Detection System (SDS) software, version 2.1.0 and Graph Pad Prism, version 5.

Results: In this study, we observed the increased VEGF-A mRNA level in adenoma polyps compared to the control group. However؛ this difference was not significant (P>0.05), and also found overexpression of VEGF-A mRNA in adenoma polyps located in the colon site in comparison to the rectum site. But statistically, these differences in VEGF-A mRNA expression did not reach to a significant level(P>0.05).

Conclusion: Adenoma polyps have more malignancy potential for making colorectal cancer and the overexpression of VEGF-A gene perhaps has a critical role in adenoma polyps malignancy process. Also, maybe the location of adenoma polyps has a important  effect on VEGF-A gene expression.