Effects of Crocin on Lerning, Spatial Memory Impairment and Necrosis Cells Death in Rats Hippocampus Area in Methamphetamine Induced Neurotoxicity
Authors
-
Monire Shafahi1
1- Dept. of Biology, PhD Candidat, Damghan Branch, Islamic Azad University, Damghan, Iran.
http://orcid.org/0000-0002-4340-7893
- Golamhassan Vaezi2 2- Dept. of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
- Hooman Shajiee3 3- Dept. of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
- Shahram Sharafi4 4- Dept. of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
-
Mehdi Khaksari*5
5- Physiology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
http://orcid.org/0000-0002-2240-1521
DOI:
https://doi.org/10.22100/jkh.v14i1.2129Keywords:
Crocin, Methamphetamine neurotoxicity, Spatial memory, Cell death.Abstract
Introduction: Methamphetamine (METH), as a highly neurotoxic compound, is associated with irreversible brain cell damage and results in neurological and psychiatric abnormalities. This study aimed to determine if crocin can protect hippocampus against METH-induced neurotoxicity.
Methods: thirty-six male Wistar rats that weighed 260-300 grs were randomly allocated to five groups of control (n=6), crocin 90 mg/kg (n=6), METH (n=6), METH + crocin 30 mg/kg (n=6), METH + crocin 60 mg/kg (n=6), and METH + crocin 90 mg/kg (n=6). METH neurotoxicity was induced by 40 mg/kg of METH in four injections (e.g., 4×10 mg/kg q. 2 h, IP). Crocin was intraperitoneally (IP) injected at 30 min, 24 h, and 48 h after the final injection of METH
Seven days after METH injection, spatial memory test was evaluated by Morris water maze and then the rats’ brains were removed for Nissl staining staining to assess necrosis neuronal death within the hippocampal CA1 area.
Results: Crocin treatment could significantly improve spatial memory deficits and reduced necrosis cell death in CA1 area of hippocampus
Conclusion: Crocin exerts neuroprotective effects on METH neurotoxicity via the inhibition of necrosis.
References
Schep LJ, Slaughter RJ, Beasley DM. The clinical toxicology of metamfetamine. Clin Toxicol 2010;48:675-94. doi: 10.3109/15563650.2010.516752
Noorbakhshnia M, Nazarizadeh Dehkordi N, Beheshti S. Protective effect of verapamil on learning and memory deficits induced by methamphetamine in rat. Ir J Physiol Pharmacol 2018;2:51-8. doi: 10.1016/j.physbeh.2018.06.016
Abe K, Saito H . Effects of saffron extract and its constituent crocin on learning behaviour and long-term potentiation. Phytother Res 2000;14:149-52. doi:10.1002/(SICI)1099-1573(200005)14:33.3.CO;2-X
Yong-qiu Z, Jian-xun L, Jan-nong W. Effects of crocin on reperfusion-induced oxidative/nitrative injury to cerebral microvessels after global cerebral ischemia. Brain Res 2007;1138:86-94. doi: 10.1016/j.brainres.2006.12.064
Hensel A, Niehues M, Lechtenberg M, Quandt B, Schepmann D, Wunsch B. Analytical and functional aspects on saffron from Crocus sativus L.: development of quality control methods,species assortment and affinity to sigma-1 and NMDA receptors. Planta Med 2006;72:1005. doi: 10.1055/s-2006-949874
Hart CL, Marvin CB, Silver R, Smith EE. Is cognitive functioning impaired in methamphetamine users? a critical review. Neuropsychopharmacology 2012;37:586-608. doi: 10.1038/npp.2011.276
Rendell PG, Mazur M, Henry JD. Prospective memory impairment in former users of methamphetamine. Psychopharmaco 2009;203:609-16. doi: 10.1007/s00213-008-1408-0
Scott JC, Woods SP, Matt GE, Meyer RA, Heaton RK, Atkinson JH, et al. Neurocognitive effects of methamphetamine: a critical review and meta-analysis. Neuropsycho review 2007;17:275-97. doi: 10.1007/s11065-007-9031-0
Bowyer JF, Clausing P, Gough B, Slikkert W Jr, Holson RR. Nitric oxide regulation of methamphetamine-induced dopamine release in caudate/putamen,” Brain Res1995;699:62-70. doi: 10.1016/0006-8993(95)00877-s
Cadet J L, Brannock C. Free radicals and the pathobiology of brain dopamine systems,” Neurochemistry International 1997;32:117-31.
Jayanthi S, Deng X, Noailles PAH, Ladenheim B, Cadet JL. Methamphetamine induces neuronal apoptosis via cross-talks between endoplasmic reticulum and mitochondriadependent death cascades,”TheFASEB Journal 2004;18:238-51. doi: 10.1096/fj.03-0295com.
Jayanthi S, McCoy MT, Beauvais G, Ladenheim B, Gilmore K, Wood W, et al. Methamphetamine induces dopamine D1 receptor-dependent endoplasmic reticulum stress-related molecular events in the rat striatum. PLoS One 2009;4:e602. doi: 10.1371/journal.pone.0006092
Hosseinzadeh H, Abootorabi A, Sadeghnia HR. Protective Effect of Crocus Sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate-Induced DNA Damage in Mice Organs. DNA Cell Biol 2008;27:657-64. doi: 10.1089/dna.2008.0767
Hosseinzadeh H, Sadeghnia HR, Ziaee T, Danaee A. Protective effect of aqueous saffron extract (Crocus sativus L.) and crocin, its active constituent, on renal ischemia reperfusioninduced oxidative damage in rats. J Pharm Pharm Sci 2005;8:387-93. doi: 10.1089/dna.2008.0767
Hosseinzadeh H, Sadeghnia HR . Safranal, a constituent of Crocus sativus (saffron) attenuated cerebral ischemia induced oxidative damage in rat hippocampus. J Pharm Pharm Sci 2005; 8:394-97.
Goyal SN, Arora S, Sharma AK, Joshi S, Ray R, Bhatia J, Kumari S, Arya DS. Preventive effect of crocin of Crocus sativus on hemodynamic, biochemical, histopathological andultrastuctural alterations in isoproterenol-induced cardiotoxicity in rats. Phytomedicine 2010;17:227-32. doi: 10.1016/j.phymed.2009.08.009
Noorbala AA, Akhondzadeh S, Tahmacebipour N, Jamshidi AH. Hydro-alcoholic extract of Crocus sativus L. versus fluoxetine in the treatment of mild to moderate depression: a doubleblind, randomized pilot trial. J Ethnopharmacol 2005;97:281-4. doi: 10.1016/j.jep.2004.11.004
Takashi O, Hiroshi S, Ken-ichi M, Katsunori I, Michihiro F, Hiroyuki T, Yukihiro S, Akihisa T, Reiko E, Shinji S. Protective effects of carotenoids from saffron on neuronal injury in vitroand in vivo. Biochimica et Biophysica Acta 2007;1770:578-584. 10.1016/j.bbagen.2006.11.012. doi: 10.1016/j.bbagen.2006.11.012
Saleem S, Ahmad M, Ahmad AS, Yousuf S, Ansari MA, Khan MB, Ishrat T, IslamF. Effect of saffron (Crocussativus) on neurobehavioral and neurochemical changes in cerebral ischemia in rats. J. Med. Food 2006;9:246–253. doi: 10.1089/jmf.2006.9.246
Soetens E, Casaer SD, Hooge R, Hueting JE. Effect of amphetamine on long-term retention of verbal material. Psychopharmaco 1995;119:155-162.
Nater U M, Okere U, Stallkamp R, Moor C, Ehlert U, Kliegel M. Psychosocial stress benhances time- based prospective memory in healthy young men. Neurobiol Learn Mem 2006;86:344-348. doi: 10.1016/j.nlm.2006.04.006
Goyal SN, Arora S, Sharma AK, Joshi S, Ray R, Bhatia J, Kumari S, Arya DS. Preventive effect of crocin of Crocus sativus on hemodynamic, biochemical, histopathological and ultrastuctural alterations in isoproterenol-induced cardiotoxicity in rats. Phytomedicine 2010;17:227-32. doi: 10.1016/j.phymed.2009.08.009
Jin hee L, Dong-hyun K. Antihyperlipidemic Effect of Crocin Isolated from the Fructus of Gardenia jasminoides and Its Metabolite Crocetin. Biol. Pharm. Bull 2005;28:2106-10. doi: 10.1248/bpb.28.2106
Hosseinzadeh H, Modaghegh MH and Saffari Z. Crocus Sativus L. (Saffron) Extract and itsActive Constituents(Crocin and Safranal) on Ischemia-Reperfusion in Rat Skeletal Muscle.Evid Based Complement Alternat Med 2009;6:343-50. doi: 10.1093/ecam/nem125
Oussama A, Rubio-Moraga A. Saffron: Its Phytochemistry, Developmental Processes, and Biotechnological Prospects. J Agric Food Chem.2015;6340:8751-8764. doi: 10.1021/acs.jafc.5b03194
S, Ken-ichi M, Katsunori I, Michihiro F, Hiroyuki T, Yukihiro S, Akihisa T, Reiko E, Shinji S. Protective effects of carotenoids from saffron on neuronal injury in vitro and in vivo. Bioch et Biophy Acta 2007;1770:578-84. doi: 10.1016/j.bbagen.2006.11.012
Yong-qiu Z, Jian-xun L, Jan-nong W. Effects of crocin on reperfusion-induced oxidative/nitrative injury to cerebral microvessels after global cerebral ischemia. Brain Res 2007;1138: 86–94. doi: 10.1016/j.brainres.2006.12.064
Hosseinzadeh H, Sadeghnia HR. Safranal, a constituent of crocus sativus (saffron) attenuated cerebral ischemia induced oxidative damage in rat hippocampus. J Pharm Pharm Sci 2005;8:394-9. doi: 10.1093/ecam/nem125
Kaushal N, R Matsumoto R. Role of sigma receptors in methamphetamine-induced neurotoxicity. Curr Neuropharmacol 2011;9:54-7. doi: 10.2174/157015911795016930
Hart CL, Marvin CB, Silver R, Smith EE. Is cognitive functioning impaired in methamphetamine users? a critical review. Neuropsychopharmaco2012;37:586-608. doi: 10.1038/npp.2011.276
Adriani W, Felici A, Sargolini F, Roullet P, Usiello A, Oliverio A, et al. N-methyl-D-aspartate and dopamine receptor involvement in the modulation of locomotor-activity and memory processes. Exp Brain Res 1998;123:52-9. doi: 10.1007/s002210050544
Thanos PK, Kim R, Delis F, Rocco MJ, Cho J, Volkow ND. Effects of chronic methamphetamine on psychomotor and cognitive functions and dopamine signaling in the brain. Behav Brain Res 2017;90:282-90. doi: 10.1016/j.bbr.2016.12.010
Murnane KS, Perrine SA, Finton BJ, Galloway MP, Howell LL, Fantegrossi WE. Effects of exposure to amphetamine derivatives on passive avoidance performance and the central levels of monoamines and their metabolites in mice: correlations between behavior and neurochemistry. Psychopharmaco 2012;220:495-508. doi: 10.1007/s00213-011-2504-0
Hensel A, Niehues M, Lechtenberg M, Quandt B, Schepmann D ,Wunsch B. Anlytical and functional aspects on saffron from crocus sativus L.: development of quality control methods, species assortment and affinity to sigma-1 andNMDA receptors. Planta Med 2006;72:1005. doi: 10.1055/s-2006-949874
Abe K, Sugiura M, Shoyama Y, Saito H. Crocin antagonizes ethanolinhibition of NMDAreceptor-mediated responses in rat hippocampal neurons. Brain Res 1998;787:132-8. doi: 10.1016/s0006-8993(97)01505-9
Abe K, Sugiura M, Yamaguchi S, Shoyama Y, Saito H. Saffron extract prevents acetaldehyde-induced inhibition of long-term potentiation in the rat dentate gyrus in vivo. Brain Res 1999;851:287-9. doi: 10.1016/s0006-8993(99)02174-5
Won S, Hong RA, Shohet RV, Seto TB, Parikh NI. Methamphetamine‐associated cardiomyopathy. Clin Cardiol 2013;36:737-42. doi: 10.1002/clc.22195
Kishi T, Tsunoka T, Ikeda M, Kitajima T, Kawashima K, Okochi T, et al. Serotonin 1A receptor gene is associated with Japanese methamphetamine-induced psychosis patients. Neuropharmacology 2010;58:452-6. doi: 10.1016/j.neuropharm.2009.09.006
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