Evaluation of the Relationship between IL4 Gene Polymorphisms (rs2227284 and rs2243250) and the Risk of Prostate Cancer in Iranian Men

Abstract

Introduction: Inflammation in tissues is one of the factors associated with cancer. Interleukins secreted by helper T cells such as IL4 with a dual role can be effective in cancer. In this study, the relationship between rs2227284 (-33T / G) and rs2243250 (-589C / T) polymorphisms of the interleukin IL4 gene and the risk of prostate cancer was investigated.

Methods: 100 blood samples from patients with prostate cancer and 100 samples from healthy individuals in the same age range were selected after approval by a specialist. The DNA of the samples was extracted by salting out method and their purity was confirmed by spectrophotometer and gel. Then, the polymorphisms in the gene were examined using RFLP-PCR technique. The results were analyzed using SPSS software version 21 with chi-square and logistic regression tests.

Results: CC mutant genotype in rs2227284 polymorphism showed a significant relationship between cancer and healthy groups. (P.V: 0.001, OR: 2.976, CI95%: 1.569-5.644). Also, both this polymorphisms with age over 60 years, body mass index above 25 had a statistically significant relationship but did not show a relationship with smoking. None of the polymorphisms were associated with cancer stage and Gleason score.

Conclusion: The rs2227284 polymorphism was associated with a higher risk of prostate cancer, and both polymorphisms were significantly associated with prostate cancer in those over 60 years of age and those with a higher body mass index. Confirmation of this requires further studies in a larger population.