Comparison of Derivation and Successful Purification of Human Adipose and Bone Marrow-Mesenchymal Stem Cells

Authors

  • Hojjat Naderi-Meshkin1 1- Dept. of Biology, School of Basic Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
  • Maryam Moghaddam Matin1
  • Naghmeh Ahmadiankia3 3- Dept. of Medicine, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
  • Asieh Heirani Tabasi2 2- Dept. of Stem Cells and Regenerative Medicine, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran.
  • Mahdi Mirahmadi2
  • Mina Shahryari2
  • Malihe Hasanzadeh2
  • Mahmoud Mahmoudi4 4- Dept. of Medicine, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
  • Nasser Sanjar Moussavi5 5- Immunology Research Centre, Bu-Ali Research Institute, Mashhad University of Medical Science, Mashhad, Iran.
  • Armin Attaranzadeh6 6- Dept. of Surgery, School of Medicine, Islamic Azad University-Mashhad Branch, Iran.
  • Ahmad Reza Bahrami1

DOI:

https://doi.org/10.22100/jkh.v9i4.320

Keywords:

Mesenchymal stem cells, Adipose tissue, Bone marrow, Derivation and expansion.

Abstract

Introduction: Mesenchymal stem cells (MSCs) exhibit multiple beneficial properties in treatment of various diseases through their differentiation capacity and secretion of paracrine signals. Therefore, using MSCs as a tool for cell therapy rely on the ability of harvesting a sufficient number of cells in a short time.

Methods: In this study, MSCs derived from bone marrow (BM) and adipose tissue (AD) were investigated in terms of success rate of culture and cell yields. To ensure that the isolated cells are MSCs, adipogenic and osteogenic differentiation study were performed by Oil Red O, Alizarin Red S and Alkaline Phosphatase assay, and specific surface markers expression examined by flow cytometry.

Results: The results showed that successful culture's rate of AD-MSCs were superior to BM-MSCs. AD-MSCs reached to confluency and passage in a shorter time (faster) and produced further amount of cells at a defined time. It was also demonstrated that the derivation of MSCs from adipose tissue were completely reproducible and not donor dependent rather than BM-MSCs.

Conclusion: In conclusion, with our protocol, adipose tissues are proposed the more appropriate source for derivation of MSCs in terms of percentage of cells successfully isolated and subsequent purification as compared to bone marrow.

Author Biography

  • Hojjat Naderi-Meshkin1, 1- Dept. of Biology, School of Basic Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
    استاديار

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Published

2014-09-21

Issue

Vol 9, No 4: 2015

Section

Original Article(s)

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

Comparison of Derivation and Successful Purification of Human Adipose and Bone Marrow-Mesenchymal Stem Cells. (2014). Knowledge and Health in Basic Medical Sciences, 9(4), Page:38-45. https://doi.org/10.22100/jkh.v9i4.320

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