کورکومین از مرگ سلولی ایجاد شده به واسطه 6-OHDA و اختلال مسیرERK در سلول‌های نوروبلاستومای انسانی جلوگیری می‌کند

Maryam Moosavi 0000-0001-6943-477X
, Maryam Owjfard
, Majid Reza Farokhi
Shiraz University of Medical Sciences

چکیده


مقدمه: کورکومین، یک ماده طبیعی شیمیایی فنولی زرد رنگ مشتق شده از زردچوبه، اثرات محافظتی در مدل‌های حیوانی و سلولی بیماری پارکینسون دارد. مطالعه حاضر با هدف بررسی اینکه آیا کورکومین مانع از اثر6-OHDA در سلول‌های نوروبلاستومای انسانی و سیگنالینگ MAPK می‌شود انجام شد.

مواد و روش‌ها: سلول‌های SH-SY5Y به مدت 24 ساعت با6-OHDA  (50 میکرومتر) درمان شدند. اثر کورکومین (2 و 5/2 میکرومولار) بر روی بقاء سلولی (MTT) و MAPK ها (ERK، JNK و p38) (آزمون وسترل بلات) مورد بررسی قرار گرفت.

نتایج: 6-OHDA باعث کاهش قابل ملاحظه‌ای از بقاء نورونی و کاهش p-ERK شد. نتایج نشان می‌دهد که کورکومین می‌تواند تا حدودی در برابر مرگ سلولی ناشی از6-OHDA و کاهش p-ERK جلوگیری کند.

 نتیجه‌گیری: این یافته‌ها پیشنهاد می‌کنند که بازگرداندن ERK به اثر محافظتی کورکومین در برابر 6-OHDA در سلول‌های نوروبلاستوما انسان مربوط است.


واژه های کلیدی


6-OHDA، پارکینسون، SH-SY5Y ، کورکومین، ERK.

تمام متن:

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DOI: 10.22100/jkh.v13i3.1925

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