The Effect of a Bout of Resistance Exercise on 8-Hydroxy-2’-Deoxyguanosine in Athletes and Non-Athletes
DOI:
https://doi.org/10.22100/jkh.v7i1.69Keywords:
Resistance exercise, 8-hydroxy-2-deoxyguanosine, Bodybuilding athletes.Abstract
Introduction: As a result of the natural metabolism in cells, free radicals and reactive species of oxygen are continuously produced and increase under physical and psychological stress conditions. Oxidative stress is a condition in which the reactive oxygen species production exceeds the antioxidant system capacity to neutralize these peroxidants. In these situations, proteins, lipids, and nucleic acids are damaged. Therefore, the purpose of this study was to determine the effects of acute resistance exercise (RE) on 8-hydroxy-2’-deoxyguanosine (8-OHdG), marker of DNA oxidation.
Methods: In this quasi-experimental research, nine resistances trained (RT) and nine untrained (UT) men were randomly selected. All subjects performed a RE protocol including 4 sets of the bench press, leg press, sited bar shoulder press, arm curls and lat pull down exercises using high intensity (80% of 1RM). Blood draws occurred before and immediately after exercise and urine samples were collected before, immediately after, 3h after and 24 h after resistance exercise for the measurement of plasma lactate and urinary 8-OHdG excretion.
Results: Urinary 8-OHdG concentrations were significantly lower at post and 24h post exercise in RT compared with UT (P<0.05). The concentration of 8-OHdG was significantly elevated post, 3h post and 24h post exercise in UT as compared with pre exercise status (P<0.05). However, 8-OHdG was significantly elevated 24h post exercise in RT as compared with pre exercise status (P<0.05).
Conclusion: The findings of the present study indicated that oxidative DNA damage was lower in bodybuilding athletes compared to the untrained. This may be due to regular resistance training status in athletes and it is possible that antioxidant capacity is improved in athletes due to performing regular resistance training.
References
Sen CK, Rankinen T, Vaisanen S, Rauramaa R. Oxidative stress after human exercise: Effect of N- acetylcysteine supplementation. J Appl Physiol 1994;76(6):2570-2577.
Halliwell B. Free radicals and antioxidants: a personal view. Nutr Rev 1994;52(8):253-265.
Wu LL, Chiou CC, Chang PY, Wu JT. Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clin Chim Acta 2004;339(1-2):1-9.
Dernbach AR, Sherman WM, Simonsen JC, Flowers KM, Lamb DR. No evidence of oxidative stress during high intensity rowing training. J Appl Physiol 1993;74(5):2140-2145.
Niess AM, Hartmann A, Grunert-Fuchs M, Poch B, Speit G. DNA damage after exhaustive treadmill running in trained and untrained men. Int J Sports Med 1996;17(6):397-403.
Konig D, Wagner KH, Elmadfa I, Berg A. Exercise and oxidative stress: significance of antioxidants with reference to inflammatory, muscular, and systemic stress. Exerc Immunol Rev 2001;7:108-133.
Clarkson PM, Thompson HS. Antioxidants: what role do they play in physical exercise and health? Am J Clin Nutr 2000;72(2):637-646.
Ji LL. Free radicals and antioxidants in exercise and sports. In: Garrett WE, Kirkendall DT, editors. Exercise and Sport Science. Lippincott Williams and Wilkin, Philadelphia; 2000.p. 299-317.
Smith LL, Miles MP. Exercise-induced muscle injury and inflammation. In: Garrett WE, Kirkendall, editors. Exercise and Sport Science. Lippincott Williams and Wilkin, Philadelphia;2000. p.401-411.
Reichhold S, Neubauer O, Bulmer AC, Knasmu S, Wagner KH. Endurance exercise and DNA stability: Is there a link to duration and intensity? Mutation Research 2009;682(1):28-38.
Bloomer RJ, Goldfarb AH. Anaerobic exercise and oxidative stress: a review. Can J Appl Physiol 2004;29(3):245-63.
Bloomer RJ, Goldfarb AH, Wideman L, Mckenzie MJ, Consitt LA. Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress. J Strength Cond Res 2005;19(2):276-285.
Kanter MM, Lesmes GR, Kaminsky LA, Ham-Saeger JL, Nequin ND. Serum creatine kinase and lactate dehydrogenase changes following an eighty kilometer race. Eur J Appl Physiolo 1988;57(1):60-63.
Morillas-Ruiz J, Zafrilla P, Almar M, Cuevas M, Lo´pez F, Abellan P, et al. The effects of an antioxidant-supplem ented beverage on exercise-induced oxidative stress: results from a placebo-controlled double-blind study in cyclists. Eur J Appl Physiol 2005;95(5-6):543-549.
Orhan H, Van Holland B, Krab B, Moeken J, Vermeulen NP, Hollander P, et al. Evaluation of a multi-parameter biomarker set for oxidative damage in man: increased urinary excretion of lipid, protein and DNA oxidation products after one hour of exercise. Free Radic Res 2004;38(12):1269-1279.
Radak Z, Pucsok J, Mecseki S, Csont T, Ferdinandy P. Muscle soreness-induced reduction in force generation is accompanied by increased nitric oxide content and DNA damage in human skeletal muscle. Free Radic Biol Med 1999;26(7-8):1059-1063.
Parise G, Brose AN, Tarnopolsky MA. Resistance exercise training decreases oxidative damage to DNA and increases cytochrome oxidase activity in older adults. Exp Gerontol 2005;40(3):173-180.
Rahimi R, Boroujerdi SS, Ghaeeni S, Noori R. The effect of different rest intervals between sets on the training volume of male athletes. Facta Univ Phys Educ Sport 2007;5(1):37-46.
Rahimi R, Qaderi M, Faraji H, Boroujerdi SS. Effects of very short rest periods on hormonal responses to resistance exercise in men. J Strength Cond Res 2010;24(7):1851-1859.
Alessio HM, Goldfarb AH. Lipid peroxidation and scavenger enzymes during exercise: adaptive response to training. J Appl Physiol 1988;64(4):1333-1336.
Davies KJ, Quintanilha AT, Brooks GA, Packer L. Free radicals and tissue damage produced by exercise. Biochem Biophys Res Commun 1982;107(4):1198-1205.
Djuric Z, Heilbrun LK, Simon MS, Smith D, Luongo DA, LoRusso PM, et al. Levels of 5-hydrox- ymethyl-2V-deoxyuridine in DNA from blood as a marker of breast cancer. Cancer 1996;77(4):691-6 .
Matsui A, Ikeda T, Enomoto K, Hosoda K, Nakashima H, Omae K, et al. Increased formation of oxidative DNA damage, 8-hydroxy-2-deoxyguanosine, in human breast cancer tissue and its relationship to GSTP1 ad COMT genotypes. Cancer Lett 2000;151(1):87-95.
Erhola M, Toyokuni S, Okada K, Tanaka T, Hiai H, Ochi H, et al. Biomarker evidence of DNA oxidation in lung cancer patients: association of urinary 8- hydroxy-2-deoxyguanosine excretion with radiotherapy, chemotherapy, and response to treatment. FEBS Lett 1997;409(2):287-91.
Chiou CC, Chang PY, Chan EC, Wu TL, Tsao KC, Wu TJ. Urinary 8-hydroxydeoxyguanosine and its analogues as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers. Clin Chim Acta 2003;334(1-2):87-94.
Kanauchi M, Nishioka H, Hashimoto T. Oxidative DNA damage and tubulointerstitial injury in diabetic nephropathy. Nephron 2002;91(2):327-9 .
Atalay M, Laaksonen DE. Diabetes, oxidative stress and physical exercise. J Sport Sci Med 2002;1:1-14.
Pilger A, Germadnik D, Formanek D, Zwick H, Winkler N, Rudigger HW. Habitual long-distance running does not enhance urinary excretion of 8-hydroxydeoxyguanosine. Eur J Appl Physiol Occup Physiol 1997;75(5):467-469.
Uchiyama S, Tsukamoto H,Yoshimura S, Tamaki T. Relationship between oxidative stress in muscle tissue and weight-lifting-induced muscle damage. Pflugers Arch - Eur J Physiol 2006;452(1):109-116.
Tamaki T, Uchiyama S, Tamura T, Nakano S. Changes in muscle oxygenation during weight-lifting exercise. Eur J Appl Physiol Occup Physiol 1994;68:465-469.
Radak Z, Naito H, Kaneko T, Tahara S, Nakamoto H, Takahashi R, Cardozo-Pelaez F, Goto S. Exercise training decreases D NA damage and increases DNA repair and resistance against oxidative stress of proteins in aged rat skeletal muscle. Pflug Arch 2002;445(2):273-278.
Robertson JD, Maughan RJ, Duthie GG, Morrice PC. Increased blood antioxidant systems of runners in response to training load. Clin Sci 1991;80(6):611-118.
Kürkçü R, Tekin A, Özda S, Akçakoyun F. The effects of regular exercise on oxidative andantioxidative parameters in young wrestlers. Afr J Pharm Pharmaco 2010;4(5):244-251.
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