The Simultaneous Effect of Regular Exercise and Vitamin D on Apoptosis Level and Antioxidant Enzymes of Heart Tissue of Male Rats Exposed to Oxygenated Water
Authors
- Mehdi Pirooz1 1- Dept. of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
-
Mohammad Ali Azarbayjani1
http://orcid.org/0000-0002-3502-7487
- Seyed Ali Hosseini 2 2- Dept. of Sport Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.
- Maghsoud Peeri1
DOI:
https://doi.org/10.22100/jkh.v13i2.1935Keywords:
Oxidative stress, Regular exercise activity, Malondialdehyde, SOD, CAT.Abstract
Introduction: Biological effects of strong oxidizing compounds in the human body are controlled by antioxidant agents. Disorders of the organs may be result of reactions between free radicals and cells membrane. It has been shown that the main objective of the oxygen radicals is the lipids cell membrane. The aim of this study was to evaluate the myocardial cellular vulnerability and the evaluation of antioxidant enzymes in the heart tissue of male rats.
Methods: Sixty adult male wistar rats, 220 ± 220 gram and 8-10 weeks years old, were randomly assigned into 10 groups of six by considering stress, vitamin D and regular exercise activities. Groups were control, water Oxygenated water H2O2 (H); twice Oxygen water of 2H2O2 (2H), Oxygen water + Vitamin D (HD), Oxygen water doubles + Vitamin D (2HD), Oxygenated water + Exercise (HE), Oxygenated water twice + Exercise (2HE)), Oxygenated Water + Exercise + Vitamin D (HDE), Oxygenated Water + Double Exercise + Vitamin D (2HDE) and finally Diethyl Sulfoxide + Saline (DMSO) were subjected to an intervention protocol for 8 weeks. Then, the concentration of antioxidant enzymes in measuring heart tissue analysis of variance was analyzed by the three-way anova. The significance level was set at 0.05.
Results: The results showed that exercise increased SOD and GPX, but catalase increase was not significant. Also, exercise interaction with vitamin D also increased the antioxidant enzymes, but these enzymes did not increase in groups which did not complete the exercise. On the other hand the concentration of malondialdehyde MDA increased in these groups. The combination of exercise, vitamin D and oxygenated water has a synergistic effect on inhibiting the activity of the FAS protein, and is effective in protect and controlling the complications of apoptosis and myocardial inflammation.
Conclusion: Concerning the use of different doses of H2O2 in comparison with the training group and D alone or in the control group, it was shown that the combination of endurance training and D increased significantly the activity of SOD and GPX enzymes.
References
Reference
Dill DB, Costill DL. (1974). Calculation of percentage changes of blood, plasma and red cells in hydration. J Appl Physiol. 37: 247-248.
Elmore S. (2007). Apoptosis: A review of programmed cell death. Toxicol Pathol. 35: 495-516.
Hunter GR, Wetzstein CJ, Fields DA, Brown A, & Bamman M. (2000). Resistance training increases total energy expenditure and free-living physical activity in older adults. J Appl Physiol. 89: 977–984.
Kruger K, Frost S, Most E, Volker K, Pallauf J, Mooren FC.(2009). Exercise affects tissue lymphocyte apoptosis via redox-sensitive and Fas-dependent signaling pathways. Am J Physiol Regul Integr Comp Physiol. 296: R1518–R1527.
Mooren FC, Blöming D, Lechtermann A, Lerch MM, Volker K. (2002). Lymphocyte apoptosis after exhaustive and moderate exercise. J Appl Physiol. 93: 147–153.
Navalta JW, Sedlock DA, Park KS. (2007). Effect of exercise intensity on exercise induced lymphocyte apoptosis. Int J Sports Med. 28: 539–542.
Jackson MJ, Pye D, Palomero J. The production of reactive oxygen and nitrogen species by skeletal muscle. J Appl Physiol 2007; 102(4):1664–1670.
Halliwell B, Cross CE. Oxygen‐derived species: Their relation to human disease and environmental stress. Environ Health Perspect 1994; 102(Suppl 10):5–12.
Bloomer RJ, Goldfarb AH. Anaerobic exercise and oxidative stress: A review. Can J Appl Physiol 2004; 29(3):245–263.
Haycock JW, Jones P, Harris JB, Mantle D. Differential susceptibility of human skeletal muscle proteins to free radical induced oxidative damage: A histochemical, immu-nocytochemical and electron microscopical study in vitro. Acta Neuropathol 1996; 92 (4):331–340.
Mooren FC, Blöming D, Lechtermann A, Lerch MM, Volker K. (2002). Lymphocyte apoptosis after exhaustive and moderate exercise. J Appl Physiol. 93: 147–153.
Volker K, Bischoff A, Agnischock S, Lechtermann A, Kruger K, Mooren FC. (2007). Lymphocyte and Granulocyte Apoptosis after Exhaustive and Moderate Resistance Training. Medicine and Science in Sports and Exercise. 39: S172.
Mooren FC, Lechtermann A, Lker K. (2004). Exercise-Induced Apoptosis of Lymphocytes Depends on Training Status. Medicine and Science in Sports and Exercise. 36: 1476-1483.
Bloomer R J.EFFECT OF EXERCISE ON OXIDATIVE STRESS BIOMARKERS. ADVANCES IN CLINICAL CHEMISTRY, 2008,VOL. 46.
Hunter GR, Wetzstein CJ, Fields DA, Brown A, & Bamman M. (2000). Resistance training increases total energy expenditure and free-living physical activity in olderadults. J Appl Physiol. 89: 977–984.
Zoppi CC, Macedo DV. Overreaching‐induced oxidative stress, enhanced HSP72 expression, antioxidant and oxidative enzymes downregulation. Scand J Med Sci Sports 2007.
Schippinger G, Wonisch W, Abuja PM, Fankhauser F, Winklhofer‐Roob BM,Halwachs G. Lipid peroxidation and antioxidant status in professional American football players during competition. Eur J Clin Invest 2002; 32(9):686–692.
Hoffman JR, Im J, Kang J, Maresh CM, Kraemer WJ, French D, et al. Comparison of low‐ and high‐intensity resistance exercise on lipid peroxidation: Role of muscle oxygenation. J Strength Cond Res 2007; 21(1):118–122.
Bloomer RJ, Goldfarb AH, Wideman L, McKenzie MJ, Consitt LA. Effectss of acute aerobic and anaerobic exercise on blood markers of oxidative stress. J Strength Cond Res 2005; 19(2):276–285
Araceli BP. (2008). Exercise as the Cornerstone of Cardiovascular Prevention. Revista Española de
Cardiología (English Edition). 61 (5): 514-528.
Aguilo A, Tauler P, Guix MP, Jeffrey BB, Susan AM. (2003). Effect of exercise intensity and training on
antioxidants and cholesterol profile in cyclists. The Journal of Nutritional Biochemistry. 14 (6): 319-325.
Ognovszky H. Berkers I, Kumagia S, Teck J, Simon T. (2005). The effects of moderate-, strenuous-and
over-training on oxidative stress markers, DNA repair, and memory, in rat brain. Neurochemistry
International. 46 (8): 635-640.
Heather KV, Cheryl MB, Arthur LW, Kevin RV, Eugene B, Karen E. (2009). Effects of antioxidant
supplementation on insulin sensitivity, endothelial adhesion molecules, and oxidative stress in normalweight
and overweight young adults. Metabolism. 58 (2): 254-262.
Moffarts B, Portier K, Kirschvink N, Coudert J, N. Fellmann N. (2007). Effects of exercise and oral
antioxidant supplementation enriched in (n − 3) fatty acids on blood oxidant markers and erythrocyte
membrane fluidity in horses. The Veterinary Journal. 174 (1):113-121.
Afzalpour ME, Gharakhanlou R, Gaeini AA, Mohebbi H, Hedayati M, Khazaei M. (2008). The effects of
aerobic exercises on the serum oxidized LDL and total antioxidant capacity in non-active men. CVD
Prevention and Control. 3 (2): 77-82.
Gjertrud AT, Inga ES, Arnt ET, Idar KG, Tomas O. (2009). Endothelial Dysfunction Induced by Post-
Prandial Lipemia: Complete Protection Afforded by High-Intensity Aerobic Interval Exercise. Journal of
the American College of Cardiology. 53 (2): 200-206.
Su, S.H., Jen, C.J., Chen, H.I. (2011). NO signaling in exercise training-induced anti-apoptotic effects in humanneutrophils. Biochemical and Biophysical Research Communications. 405(1): 58-63.
Baek, S.S., Jun, T.W., Kim, K.J., Shin, M.S., Kang, S.Y., Kim, C.J. (2012). Effects of postnatal treadmill exercise on
apoptotic neuronal cell death and cell proliferation of maternal-separated rat. pups. Brain & Development
(1): 45-56.
Mirdar, S., Arab, A., Hajizade, A., Hedayati, M. (2012). The effect of pregnant rat swimming on hypoxia-inducible
factor-1α levels of neonatal lung. Tehran Univ Med J. 69)12): 754-60.
Peters EM, Van Eden M, Tyler N, Ramautar A, Chuturgoon AA.(2006). Prolonged
exercise does not cause lymphocyte DNA damage or increased apoptosis in welltrained
endurance athletes. European Journal of Applied Physiology. 98: 124-131.
(Abstract).
Qi Z, He J, Zhang Y, et al. Exercise trainingattenuates oxidative stress and decreases p53protein content in skeletal muscle of type 2diabetic. Free Radic Biol Med 2011; 50: 794-800.
Sun L, Shen W, Liu Z, et al. Endurance exercisecauses mitochondrial and oxidative stress in ratliver: Effects of a combination of mitochondrialtargeting nutrients. Life Sci 2010; 86: 39-44.
Hoffman-Goetz L, Pervaiz N, Guan J. Voluntaryexercise training in mice increases theexpression ofantioxidant enzymes and decreases the expressionof TNF-alpha in intestinal lymphocytes. BrainBehav Immun 2009; 23: 498-506.
Navalta JW, McFarlin BK, Lyons TS, Faircloth JC, Bacon NT, Callahan ZJ. (2009).Exercise-induced lymphocyte apoptosis attributable to cycle ergometer exercise inendurance-trained individuals. Applied Physiology, Nutrition, and Metabolism. 34:603-608. (Abstract).
Sedlock DA, Park KS, Navalta JW, McFarlin BK. (2007). Neither gender normenstrual cycle phase influences exercise-induced lymphocyte apoptosis inuntrained subjects. Applied Physiology, Nutrition, and Metabolism. 32: 481-486.
Atalay, M. and Laaksonen, D.E. (2002): Diabetes, oxidative stress and physical exercise. Journal of Sports Science and Medicine, 1: 1-14.
Doustar, Y., Salehi, I., Mohamadi, M., Mohajeri, D. and Hashemi, M. (1999): Study of effects of treadmill exercise on diabetic nephropathy in rats. Medical science journal of Islamic university Tehran Medical Unit 2007, 17: 187-193.
Fahim, M.A., El-Sabban, F. and Davidson N. (1998): Muscle contractility decrement and correlated morphology during the pathogenesis of streptozotocin-diabetic mice. Anat. Rec., 251: 240-244
Steensberg A, Morrow J, Toft AD, Bruunsgaard H, Pedersen BK. (2002). Prolongedexercise, lymphocyte apoptosis and F2-isoprostanes. Eur J Appl Physiol. 87: 38-42.
Tuan TC, Hsu TG, Fong MC, Hsu CF, Tsai KKC, Lee CY, Kong CW.(2008).Deleterious effects of short-term, high-intensity exercise on immune function:evidence from leucocyte mitochondrial alterations and apoptosis. Br J Sports Med.42: 11-15.
Wang JSh, Huang YH. (2005). Effects of exercise intensity on lymphocyteapoptosis induced by oxidative stress in men. Eur J Appl Physiol. 95: 290–297.__
Schumacher Y, Schmid A, König D, Berg A. Effects of exercise on soluble transferring receptor andother variables of the iron status. Br J Sports Med 2002; 36: 195-199.
Shemshaki A, Ghanbari N, Rajab H, Hedayati M, Salami F. Intense Alpine Skiing Exercise onAntioxidant Status of Male Skiers. Iranian Journal of Endocrinology and Metabolism 2006; 9(3): 191-197.
Mari-Carmen M, Gomez-Cabrera, Elena D, Jose V. Moderate exercise is an antioxidant: Upregulationof antioxidant genes by training. Free RadicBiol Med 2008; 44: 126–131.
Poirier B, Lannaud-Bournoville M, Conti M, Bazin R, Michel O, Bariety J, et al. Oxidative stressoccurs in absence of hyperglycemia and inflammation in the onset of kidney lesions in normotensiveobese rats. Nephrol Dial Transplant 2000; 15: 467-476.
Ascensao A, Ferreira R, Magalhaes J. Exercise-induced cardioprotection biochemical
morphological and functional evidence in whole tissue and isolated mitochondria. J physiol. 2007Apr; 117(1): 16-30
Wang GG, Li W, Lu XH, Zhao X, Xu L. Taurine attenuates oxidative stress and alleviates cardiac failure intype I diabetic rats. Croat Med J 2013; 54(2): 171-9.
Ascensão A, Magalhães J, Soares J, Ferreira R, Neuparth M, Marques F, et al. Endurance training attenuates doxorubicin-induced cardiac oxidative damage in mice. International journal of cardiology. 2005;100(3):451-60
Turgut G, Demir S, Genç O, Karabulut I, Akalin N. The effect of swimming exercise on lipid peroxidation in the rat brain, liver and heart. Actaphysiologica et pharmacologicaBulgarica. 2002;27(2-3):43-5.
Santana-Filomeno F, KormanovskiA,Hernández-Cruz T, Campos-Rodríguez R.Oxidant/antioxidant response during fasting andexhaustive swimming in the kidney of trained
mice. Journal of Cell and Animal Biology.2012; 6(12):175-81.
Swamy MS, Sivanna N, TamatamA,Khanum F. Effect of poly phenols in enhancingthe swimming capacity of rats. FunctionalFoods in Health and Disease. 2011; 1(11):482-91
Saxton JM, Nicolas E, Patrick L, Christian P. (2003). Indices of feer- radical mediated damage followingmaximum voluntary eccentric and concentric muscular work. European Jorunal of Applied physiology.
Ulrich PJ, Paolo CC, Kartikya A, Alhakam H, Duygu O, Thomas D, David SH. (2007). Exercise-InducedIncreases in Oxidized Low-Density Lipoprotein Are Associated With Adverse Outcomes in ChronicHeart Failure. Journal of Cardiac Failure. 13 (9): 759-764.
Cooke M, Iosia M, Buford T, Shelmadine B, Hudson G, Kerksick C, Rasmussen C, Greenwood M,Leutholtz B, Willoughby D, &Kreider R. Effects of acute and 14-day coenzyme Q10 supplementationon exercise performance in both trained and untrained individuals. Journal of the International Societyof Sports Nutrition 2008; 5: 8.
Kim JD, Yu BP, McCarter RJ, Lee SY, Herlihy JT. Exercise and diet modulate cardiac lipid peroxidation andantioxidant defenses. Free Radic Bio Med 1996; 20(1): 83-8.
Kon M, Tanabe K, Akimoto T, Kimura F,Tanimura Y, Shimizu K, et al. Reducingexercise-induced muscular injury in kendoathletes with supplementation of coenzymeQ10. Br J Nutr 2008; 100(4): 903-9.
Saxton J M, Donnelly A E, and Roper H P.(1994). “Indices of free-radicalmediateddamage following maximum voluntary eccentric and concentricmuscular work”. Eur. J. Appl. Physiol. 68: pp:189-193.
Mc bride JM, Kraemer W J, Triplett-Mcbride T, and SebastianelliW.(1998). “Effect of resistance exercise on free radical production”. Med.Sci. Sports Exerc. 30: pp:67-72.
Hernandez-Torres RP, Ramos-Jimenez A, Torres-Duran PV, Romero-Gonzalez J, Mascher D. (2009).Effects of single sessions of low-intensity continuous and moderate-intensity intermittent exercise onblood lipids in the same endurance runners. Journal of Science and Medicine in Sport. 12 (2):323-331.
Ziegler S, Schaller G, Mittermayer F, Assuero G, Gianmario S, Danilo N, Oberdan. (2006). Exercisetraining improves low-density lipoprotein oxidability in untrained subjects with coronary artery disease.Archives of Physical Medicine and Rehabilitation . 87 (2): 265-269
Afzalpour ME, Gharakhanlou R, Gaeini AA, Mohebbi H, Hedayati M, Khazaei M. (2008). The effects ofaerobic exercises on the serum oxidized LDL and total antioxidant capacity in non-active men. CVDPrevention and Control. 3 (2): 77-
Konstantinos M, Ioannis GF, Athanasios ZJ, Michalis GN, Ioannis D. (2007). Oxidative stress biomarkersresponses to physical overtraining: Implications for diagnosis. Free Radical Biology and Medicine. 43(6): 901-910.
Rauscher FM, Sanders RA, Watkins JB. Effects of coenzyme Q10 treatment on antioxidant pathways in normalandstreptozotocin-induced diabetic rats. J BiochemMolToxicol 2001; 15(1): 41-6.
Ouali K, Trea F, Toumi ML, Bairi M, Siaud P, Guellati M. Oxidative stress in streptozotocin-inducedexperimental diabetes in rats is associated with changes of antioxidant status of heart tissue. SciTechnologie C2007; 18-23.
Raedschelders K, Ansley DM, Chen DD. The cellular and molecular origin of reactive oxygen speciesgeneration during myocardial ischemia and reperfusion. PharmacolTher 2012; 133(2): 230–55.
Ascensão A, Magalhães J, Soares J, Oliveira J, Duarte JA. Exercise and cardiac oxidative stress. Rev PortCardiol 2003; (5):651-78.
Tsutsui H, Kinugawa S, Matsushima S, Yokota T. Oxidative stress in cardiac and skeletal muscle dysfunctionassociated with diabetes mellitus. J ClinBiocheNutr 2011; 48(1): 68-71.
Navarro AA, Sanchez–Del-pino MJ. Age and exercise-related changes in lipid peroxidation and superoxidedismutase activity in liver and soleus muscle tissue of rats. Mech ageing Dev 1998; 104: 91-102.
Gunduz F, Senturk UK, Kuru O, Aktekin B, Aktekin MR. The effect of one year swimming exercise on oxidativestress and antioxidant capacity inaged rats. Physiol Res 2004; 53: 171-76
Cholewa J, Poprzęcki S, Zajac A, Waskiewicz Z. (2008). The influence of vitamin C on blood oxidativestress parameters in basketball players in response to maximal exercise. Science & Sports. 23 (4):
Ulrich PJ, Paolo CC, Kartikya A, Alhakam H, Duygu O, Thomas D, David SH. (2007). Exercise-InducedIncreases in Oxidized Low-Density Lipoprotein Are Associated With Adverse Outcomes in ChronicHeart Failure. Journal of Cardiac Failure. 13 (9): 759-764.
Heather KV, Cheryl MB, Arthur LW, Kevin RV, Eugene B, Karen E. (2009). Effects of antioxidantsupplementation on insulin sensitivity, endothelial adhesion molecules, and oxidative stress in normalweightand overweight young adults. Metabolism. 58 (2): 254-262.
Judge S, Jang YM, Smith A, Selman C, Phillips T, Speakman JR, et al. Exercise by lifelong voluntary wheelrunning reduces subsarcolemmal and interfibrillar mitochondrial hydrogen peroxide production in the heart. AmJPhysiolRegulIntegr Comp Physiol 2005; 289(6): R1564-72.
Gaieni AA, Mogharnesi M, Goudarzi M, Soori R. The estimate of the blood lipid variables using youngstudents’ BF% and LBM indicators. Journal of sport sciences. 2005;1(2): 49-58.
Afzalpour ME, Gharakhanlou R, Gaieni AA, Mohebi H, Hedayati M. Effects of moderate and vigorousaerobic exercise on the serum arylesterase enzyme activity and total antioxidant capacity in sedentary men.2005; Research on Sport Sciences. 2005; 9: 34.
Warner DS, Sheng H, Batinić-Haberle I. Oxidants, antioxidants and the ischemic brain. Journal of experimental biology. 2004;207(18):3221-31.
Ricardo A, Pinhoab, Michael E, Andrades A, Marcos R, Oliveira Aline C,Pirola. Imbalance inSOD/CAT activities in rat skeletal muscles submitted to treadmill training exercise. Cell BiologyInternational 2006; 30: 848-853.
Dĺaz- Castro J, Guisado R, Kajarabille N, Garacia C, Guisado I, Teresa C, et al. Coenzyme Q10supplementation ameliorates inflammatory signaling and oxidative stress associated with strenuousexercise. Eur J Nutr 2011; DOI 10.1007/s00394-011-0257-5.
Vani M, GP-Reddy GR,ReddyK,Thyagraju and redanna. Glutathion-s-transferase, superoxidedismutase, xantin oxidase, catalase, glutathione proxidase and lipid peroxidation in the liver ofexercisedrats.BiochemInt 2000; 21(1):17-26.
Watson TA, MacDonald-Wicks LK, Garg ML. Oxidative stress and antioxidants in athletes undertaking regularexercise training. Int J sport NutrExercMetab 2005; 15: 131-46.
Ogonovsky H, Sasvari M, Dosek A, Berkes I, Kaneko T, Tahara S, et al. The effects of moderate, strenuous andovertraining on oxidative stress markers and DNA repair in rat liver. Can J Applphysiol 2005; 30: 186-95.
Parise G,PhillipsSM,KaczorJJ,Tarnopolsky MA.(2005). “Antioxidantenzyme activity is up- regulated after unilateral resistance exercisetraining in older adults”. Free Radic Bio Med.15,39(2): pp:289-95.
Siadat ZD, Kiani K, Sadeghi M, Shariat AS, et al. Association of vitamin D deficiency and coronary artery disease with cardiovascular risk factors. J Res Med Sci. 2012; 17(11):1052-5.
Radák Z, Sasvári M, Nyakas C, Pucsok J, Nakamoto H, GotoS.Exercise preconditioning against hydrogen peroxide-inducedoxidative damage in proteins of rat myocardium. Arch BiochemBiophys2000;376:248-51. doi: 10.1006/abbi.2000.1719
Li SF, Liu HX, Zhang YB, Yan YC, Li YP. The protective effectsof alpha-ketoacids against oxidative stress on rat spermatozoa invitro. Asian J Androl2010;12:247-56. doi: 10.1038%2Faja.2009.78
Maletic S, Dragicevic-Djokovic LM, Ognjanovic B, Zikic R, StajnA, Kostic M. Alterations of rat reticulocyte (anti) oxidant status andenergy metabolism influenced by hydrogen-peroxide. ActaBiologicaIugoslavicaSerija C 1999;35.
Makino A, Skelton MM, Zou AP, Cowley AW. Increased renalmedullary H2O2 leads to hypertension. Hypertension 2003;42:25-30.doi: 10.1161/01.HYP.0000074903.96928.91
Halder SK, Sharan C, Al-Hendy A. 1, 25-dihydroxyvitamin D3treatment shrinks uterine leiomyoma tumors in the Eker rat model.BiolReprod 2012;86:116. doi: 10.1095/biolreprod.111.098145
Husain K, Hazelrigg SR. Oxidative injury due to chronic nitricoxide synthase inhibition in rat: effect of regular exercise on theheart. BiochimBiophysActa2002;1587:75-82. doi: 10.1016/S0925-4439(02)00070-4
Plant DR, Gregorevic P, Warmington SA, Williams DA, LynchGS. Endurance training adaptations modulate the redox–forcerelationship of rat isolated slow‐twitch skeletal muscles. ClinExpPharmacolPhysiol2003;30:77-81
Baluchnejadmojarad T, Roghani M, MafakheriM.Neuroprotective effect of silymarin in 6-hydroxydopamine hemi-parkinsonian rat:involvement of estrogen receptors and oxidativestress. Neuroscilett 2010; 480(3): 206-10.
Jadhav S, Sarkar S, Tripathi H. Cytogenetic effectsof a mixture of selected metals following
subchronic exposure through drinking water inmale rats. Indian J ExpBiol 2006; 44(12): 997 -05.
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