Association of TLR4 rs4986790 and rs4986791 gene polymorphisms with risk of peptic ulcer and H.pylori infection in Guilan province population
Authors
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Seyedeh Tannaz Kamooneh
Department of Cell and Molecular Biology, Faculty of Biological Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.
https://orcid.org/0009-0006-4647-3390
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Rasoul Zahmatkesh Roodsari
- Department of Cell and Molecular Biology, Faculty of Biological Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.
https://orcid.org/0000-0003-4073-487X
DOI:
https://doi.org/10.22100/jkh.v20i1.3380Keywords:
Polymorphism, Peptic ulcer, TLR4 gene, Helicobacter pyloriAbstract
Introduction: Peptic ulcer is one of the most common gastrointestinal diseases in humans. Helicobacter pylori infection is a major cause of peptic ulcers. Toll-like receptors (TLRs) regulate innate immune signaling pathways by recognizing specific bacterial components. TLR4, in particular, activates immune responses against pathogens by recognizing lipopolysaccharides. This study investigated the effects of TLR4 rs4986790 and rs4986791 polymorphisms on susceptibility to peptic ulcers and Helicobacter pylori infection.
Methods: In this case-control study, 107 patients with peptic ulcers and 123 healthy individuals were evaluated. Genomic DNA was extracted, and the genotypic frequencies in patient and control groups were analyzed using the Tetra-primer ARMS-PCR method. Data analysis was performed using SPSS software.
Results: Based on the results, there is a statistically significant difference between the frequency of the GG genotype was observed at rs4986791 position of TLR4 gene with peptic ulcer disease and Helicobacter pylori infection among the patient and control groups.
Conclusion: Statistical analysis revealed that the G and T alleles in the TLR4 gene may serve as potential risk factors for peptic ulcer and Helicobacter pylori infection. However, further studies involving larger and more diverse populations are necessary to validate these findings.
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